The Human MT2 (Metallothionein-2) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of metallothionein-2 levels in human samples such as serum, plasma, and cell culture supernatants. Metallothionein-2 is a key protein involved in metal ion homeostasis and oxidative stress response, making it a crucial biomarker for studying conditions related to heavy metal toxicity, oxidative damage, and various diseases.
This kit offers reliable and reproducible results, making it an ideal tool for researchers and clinicians looking to investigate the role of metallothionein-2 in health and disease outcomes. With its high performance and ease of use, the Human MT2 CLIA Kit is a valuable resource for a wide range of research applications.
Product Name:
Human MT2 (Metallothionein 2) CLIA Kit
SKU:
HUES00861
Target:
Human MT2 (Metallothionein 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MT2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MT2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MT2. You can calculate the concentration of Human MT2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-116
96-108
87-102
Average (%)
109
102
95
1:4
Range (%)
85-99
88-102
91-106
Average (%)
90
94
98
1:8
Range (%)
91-103
93-108
95-110
Average (%)
97
99
101
1:16
Range (%)
86-100
94-108
100-113
Average (%)
93
100
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-113
107
EDTA plasma (n=5)
88-103
95
Cell culture media (n=5)
90-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
192.21
637.06
1964.61
203.91
601.67
1997.49
Standard deviation
16.88
46.57
163.06
16.48
71.3
182.37
C V (%)
8.78
7.31
8.3
8.08
11.85
9.13
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MT2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MT2 in samples. No significant cross-reactivity or interference between Human MT2 and analogues was observed.
