The Human MT3 (Metallothionein-3) CLIA Kit is specifically designed for the quantitative measurement of MT3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Metallothionein-3 is a key protein involved in metal homeostasis and oxidative stress response in the body. It plays a crucial role in detoxifying heavy metals, protecting against free radicals, and regulating essential metal ions.
Dysregulation of MT3 has been linked to various health conditions including neurodegenerative diseases, cancer, and metal toxicity, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.Overall, the Human MT3 CLIA Kit provides researchers with a powerful tool to accurately measure MT3 levels, furthering our understanding of its role in health and disease.
Product Name:
Human MT3 (Metallothionein 3) CLIA Kit
SKU:
HUES00405
Target:
Human MT3 (Metallothionein 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MT3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MT3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MT3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MT3. You can calculate the concentration of Human MT3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-99
88-99
99-112
Average (%)
91
93
105
1:4
Range (%)
93-107
91-107
96-107
Average (%)
99
98
101
1:8
Range (%)
95-113
88-103
96-111
Average (%)
103
94
104
1:16
Range (%)
90-104
89-106
87-97
Average (%)
95
96
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-107
101
EDTA plasma (n=5)
88-101
95
Cell culture media (n=5)
97-110
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.43
291.82
847.97
91.47
317.67
859.34
Standard deviation
7.6
32.71
63.68
8.31
27.48
52.59
C V (%)
8.31
11.21
7.51
9.08
8.65
6.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MT3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MT3 in samples. No significant cross-reactivity or interference between Human MT3 and analogues was observed.
