The Human MUC20 (Mucin 20) CLIA Kit is a reliable tool for the quantitative measurement of MUC20 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it suitable for a range of research applications.MUC20 is a mucin protein that plays a critical role in mucin production and mucosal immunity. Abnormalities in MUC20 expression have been associated with various diseases, including inflammatory bowel disease and certain types of cancer.
By measuring MUC20 levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the Human MUC20 CLIA Kit is a valuable tool for studying the role of MUC20 in human health and disease, and it can help advance research efforts aimed at developing novel treatment strategies.
Product Name:
Human MUC20 (Mucin 20) CLIA Kit
SKU:
HUES00419
Target:
Human MUC20 (Mucin 20)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC20. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC20 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC20, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MUC20. You can calculate the concentration of Human MUC20 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
103-118
86-102
Average (%)
101
109
93
1:4
Range (%)
91-105
93-109
96-111
Average (%)
98
100
103
1:8
Range (%)
92-104
92-108
100-114
Average (%)
98
99
108
1:16
Range (%)
94-108
86-101
97-109
Average (%)
102
93
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-95
90
EDTA plasma (n=5)
89-101
95
Cell culture media (n=5)
90-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.82
274.18
906.21
103.14
264.67
938.43
Standard deviation
11.11
24.98
94.16
10.77
28.88
58.46
C V (%)
10.5
9.11
10.39
10.44
10.91
6.23
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MUC20 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC20 in samples. No significant cross-reactivity or interference between Human MUC20 and analogues was observed.
