The Human MUC4 (Mucin 4) CLIA Kit is a cutting-edge assay designed for the precise measurement of MUC4 levels in human samples including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an indispensable tool for various research applications.MUC4, a membrane-bound glycoprotein, plays a crucial role in cell adhesion and signaling processes. Dysregulation of MUC4 expression has been implicated in various diseases, including cancer, inflammatory conditions, and respiratory disorders.
By accurately quantifying MUC4 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.Overall, the Human MUC4 CLIA Kit offers a reliable and efficient solution for studying the role of MUC4 in health and disease, providing researchers with a valuable tool for advancing their research in the field of mucin biology.
Product Name:
Human MUC4 (Mucin 4) CLIA Kit
SKU:
HUES00453
Target:
Human MUC4 (Mucin 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MUC4. You can calculate the concentration of Human MUC4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
96-110
85-99
Average (%)
93
102
91
1:4
Range (%)
102-116
93-109
89-103
Average (%)
108
101
95
1:8
Range (%)
94-109
93-105
92-106
Average (%)
101
100
97
1:16
Range (%)
87-99
83-95
92-108
Average (%)
94
90
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
98
EDTA plasma (n=5)
85-97
91
Cell culture media (n=5)
90-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.66
626.41
1420.5
209.12
675.43
1549.06
Standard deviation
24.29
48.11
105.69
20.79
62.27
128.73
C V (%)
11.87
7.68
7.44
9.94
9.22
8.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MUC4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC4 in samples. No significant cross-reactivity or interference between Human MUC4 and analogues was observed.
