The Human MUC6 (Mucin 6) CLIA Kit is specifically designed for the quantitative detection of Mucin 6 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and precision, providing reliable and consistent results for research purposes.Mucin 6 is a vital component of mucous membranes and plays a crucial role in protecting and lubricating the epithelial surfaces of various organs, including the gastrointestinal tract.
Abnormal levels of Mucin 6 have been associated with various pathological conditions, such as inflammatory bowel disease and gastric cancer, making it a valuable biomarker for studying these diseases and designing potential therapeutic interventions.Overall, the Human MUC6 CLIA Kit is an essential tool for researchers studying mucin biology and its role in disease pathogenesis, offering accurate and precise measurements for a wide range of applications.
Product Name:
Human MUC6 (Mucin 6) CLIA Kit
SKU:
HUES00454
Target:
Human MUC6 (Mucin 6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MUC6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MUC6. You can calculate the concentration of Human MUC6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
86-98
98-112
Average (%)
90
92
105
1:4
Range (%)
98-113
89-102
94-110
Average (%)
106
94
101
1:8
Range (%)
87-99
85-100
102-119
Average (%)
92
92
109
1:16
Range (%)
99-116
91-101
94-109
Average (%)
106
96
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
100
EDTA plasma (n=5)
88-103
94
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
220.21
550.84
1353.41
238.59
588.12
1288.89
Standard deviation
28.36
49.63
96.63
30.09
49.75
130.82
C V (%)
12.88
9.01
7.14
12.61
8.46
10.15
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MUC6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC6 in samples. No significant cross-reactivity or interference between Human MUC6 and analogues was observed.
