The Human MUC6 (Mucin 6) ELISA Kit is specifically designed for the precise measurement of Mucin 6 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for various research applications.Mucin 6 is a vital component of mucosal barriers in the body, providing protection against pathogens and maintaining tissue homeostasis. Abnormal levels of Mucin 6 have been associated with various diseases, including gastrointestinal disorders and respiratory infections, making it a valuable biomarker for studying these conditions and developing potential treatments.
Overall, the Human MUC6 (Mucin 6) ELISA Kit is a reliable tool for researchers seeking to explore the role of Mucin 6 in health and disease, offering a convenient and efficient method for quantifying this important protein in biological samples.
Product Name:
Human MUC6 (Mucin 6) ELISA Kit
SKU:
HUES01867
Target:
Human MUC6 (Mucin 6)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MUC6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MUC6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MUC6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MUC6. You can calculate the concentration of Human MUC6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
97-115
96-113
Average (%)
91
105
103
1:4
Range (%)
97-111
85-97
98-112
Average (%)
104
92
104
1:8
Range (%)
98-112
83-98
91-106
Average (%)
106
90
99
1:16
Range (%)
94-109
86-100
93-107
Average (%)
100
91
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
93
EDTA plasma (n=5)
87-100
94
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.1
7.6
1.1
1.9
7.1
Standard deviation
0.1
0.1
0.4
0.1
0.1
0.3
C V (%)
10
4.76
5.26
9.09
5.26
4.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human MUC6 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MUC6 in samples. No significant cross-reactivity or interference between Human MUC6 and analogues was observed.
