Human Myeloid Progenitor Inhibitory Factor 1 (MPIF1) ELISA Kit
The Human Myeloid Progenitor Inhibitory Factor 1 (MPIF1) ELISA Kit is a cutting-edge tool designed for the precise measurement of MPIF1 levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and dependable results, perfect for a diverse array of research studies.MPIF1, also known as CXCL9, is a key player in immune regulation, functioning as a chemoattractant for monocytes, T cells, and dendritic cells. By modulating immune responses, MPIF1 is linked to various diseases like inflammatory disorders, autoimmune conditions, and cancer, making it a valuable biomarker for investigating these pathologies and exploring potential therapeutic avenues.
Don't miss out on this invaluable tool for your research endeavors, as the Human MPIF1 ELISA Kit allows for in-depth investigations into immune modulation and disease mechanisms. Get your kit today and unlock new insights into the complex world of immune regulation.
Product Name:
Human Myeloid Progenitor Inhibitory Factor 1 (MPIF1) ELISA Kit
Product Code:
HUDL01894
Size:
96 Assays
Target:
MPIF1
Synonyms:
CCL23, SCYA23, Ckb-8, MIP-3, CKb8, Chemokine(C-C-Motif)ligand 23, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 23, Macrophage inflammatory protein 3
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
