Human MYH10 (Myosin Heavy Chain 10, Non Muscle) CLIA Kit
The Human MYH10 (Myosin Heavy Chain 10) Non-Muscle CLIA Kit is a powerful tool for the quantitative measurement of MYH10 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.MYH10 is a non-muscle myosin protein that is involved in various cellular processes, including cell migration, adhesion, and cytokinesis.
Dysregulation of MYH10 expression has been linked to diseases such as cancer, cardiovascular disorders, and neurological conditions, making it a valuable biomarker for studying these diseases and developing potential therapeutic interventions.With its reliable performance and ease of use, the Human MYH10 Non-Muscle CLIA Kit is an indispensable tool for researchers seeking to investigate the role of MYH10 in health and disease.
Product Name:
Human MYH10 (Myosin Heavy Chain 10, Non Muscle) CLIA Kit
SKU:
HUES00456
Target:
Human MYH10 (Myosin Heavy Chain 10, Non Muscle)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MYH10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MYH10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MYH10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MYH10. You can calculate the concentration of Human MYH10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
97-115
93-106
Average (%)
95
105
99
1:4
Range (%)
85-98
92-106
100-113
Average (%)
90
99
106
1:8
Range (%)
86-100
96-109
97-110
Average (%)
93
102
104
1:16
Range (%)
100-113
85-101
87-101
Average (%)
106
92
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
91
EDTA plasma (n=5)
96-111
103
Cell culture media (n=5)
94-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.83
216.35
805.12
93.87
224.66
791.66
Standard deviation
9.67
17.09
85.58
9.57
19.68
52.49
C V (%)
10.2
7.9
10.63
10.19
8.76
6.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MYH10 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MYH10 in samples. No significant cross-reactivity or interference between Human MYH10 and analogues was observed.
