Human MYL9 (Myosin Light Chain 9, Regulatory) CLIA Kit
The Human MYL9 (Myosin Light Chain 9) CLIA Kit is specifically designed for the quantitative detection of MYL9 levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.MYL9 is a regulatory protein that plays a crucial role in muscle contraction and cell motility. Abnormal levels of MYL9 have been associated with various diseases, including cardiovascular disorders, smooth muscle diseases, and cancer.
Studying MYL9 levels can provide valuable insights into the pathogenesis of these conditions and may lead to the development of targeted therapies.The Human MYL9 CLIA Kit from Assay Genie provides researchers with a powerful tool for investigating the role of MYL9 in health and disease. With its superior performance and ease of use, this kit is well-suited for a wide range of studies in the fields of molecular biology, pharmacology, and clinical research.
Product Name:
Human MYL9 (Myosin Light Chain 9, Regulatory) CLIA Kit
SKU:
HUES00458
Target:
Human MYL9 (Myosin Light Chain 9, Regulatory)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MYL9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MYL9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MYL9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human MYL9. You can calculate the concentration of Human MYL9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-113
93-110
87-98
Average (%)
103
100
92
1:4
Range (%)
86-100
100-116
84-97
Average (%)
92
106
90
1:8
Range (%)
95-108
94-109
95-110
Average (%)
101
99
101
1:16
Range (%)
90-104
87-101
86-99
Average (%)
97
94
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
100
EDTA plasma (n=5)
89-102
94
Cell culture media (n=5)
97-112
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.79
84.09
372.03
48.62
89.88
400.18
Standard deviation
5.96
6.25
27.12
5.84
8.39
38.1
C V (%)
11.51
7.43
7.29
12.01
9.33
9.52
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human MYL9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human MYL9 in samples. No significant cross-reactivity or interference between Human MYL9 and analogues was observed.
