Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
The Human Myosin Heavy Chain 1 (MYH1) ELISA Kit is specifically designed for the accurate quantitative detection of MYH1 levels in human skeletal muscle tissue samples. This kit boasts high sensitivity and specificity, providing reliable and consistent results for research purposes.Myosin Heavy Chain 1 is a key protein found in adult skeletal muscle, playing an essential role in muscle contraction and movement. Abnormal MYH1 levels have been linked to various muscular disorders and conditions, making it a valuable biomarker for studying muscle health and function.
This ELISA kit is user-friendly and efficient, allowing researchers to easily measure MYH1 levels in a variety of samples. It is a valuable tool for studying skeletal muscle physiology, monitoring muscle health in clinical settings, and exploring potential therapeutic options for muscle-related diseases.
Product Name:
Human Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1) ELISA Kit
Product Code:
HUDL01954
Size:
96 Assays
Target:
MYH1
Synonyms:
MYHSA1, MYHa, MyHC-2X/D, Myosin-1, Myosin Heavy Chain IIx/d
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
