Human Myosin Heavy Chain 11, Smooth Muscle (MYH11) ELISA Kit
The Human Myosin Heavy Chain 11 (Smooth Muscle) ELISA Kit is a cutting-edge tool designed for the precise measurement of Myosin Heavy Chain 11 levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring consistent and accurate results, making it the perfect choice for a wide array of research applications.Myosin Heavy Chain 11 is a vital protein that plays a crucial role in smooth muscle contraction and function. It is involved in various physiological processes, including muscle contraction, blood vessel tone regulation, and organ function.
Dysregulation of Myosin Heavy Chain 11 has been implicated in conditions such as hypertension, asthma, and certain vascular diseases, highlighting its importance as a biomarker for studying these diseases and potential drug development.Trust the Human Myosin Heavy Chain 11 (Smooth Muscle) ELISA Kit to provide you with reliable data and valuable insights into the role of Myosin Heavy Chain 11 in health and disease. Order yours today and take your research to the next level.
Product Name:
Human Myosin Heavy Chain 11, Smooth Muscle (MYH11) ELISA Kit
Product Code:
HUDL01953
Size:
96 Assays
Target:
MYH11
Synonyms:
AAT4, FAA4, SMHC, SMMHC, Myosin heavy chain, smooth muscle isoform
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
