Human Myosin Light Chain 2, Regulatory, Cardiac (MYL2) ELISA Kit
The Human Myosin Light Chain 2 Regulatory Cardiac (MYL2) ELISA Kit is a cutting-edge tool designed for the precise measurement of MYL2 levels in human samples, including serum, plasma, and cell culture supernatants. This advanced kit boasts exceptional sensitivity and specificity, ensuring the accurate and dependable quantification of MYL2 levels for a variety of research applications.MYL2 is a crucial regulatory protein found in cardiac muscle, playing a key role in muscle contraction and heart function. Abnormalities in MYL2 expression have been linked to various cardiovascular diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
With its reliable performance and user-friendly design, the Human MYL2 ELISA Kit from Assay Genie is the perfect solution for researchers looking to investigate the role of MYL2 in cardiovascular health and disease. Trust in the accuracy and precision of this kit to advance your research and uncover new insights into cardiac muscle function.
Product Name:
Human Myosin Light Chain 2, Regulatory, Cardiac (MYL2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
