Human Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) ELISA Kit
The Human Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS) ELISA Kit is specifically designed for the precise measurement of MARCKS levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and dependable results for a variety of research applications.MARCKS is a critical protein involved in various cellular processes such as cytoskeletal dynamics, membrane trafficking, and signaling pathways.
Dysregulation of MARCKS has been implicated in diseases like cancer, neurodegenerative disorders, and cardiovascular conditions, highlighting its importance as a biomarker for understanding and potentially treating these diseases.By utilizing the Human MARCKS ELISA Kit, researchers can gain valuable insights into the role of MARCKS in disease development and progression, ultimately paving the way for novel therapeutic interventions.
Product Name:
Human Myristoylated Alanine Rich Protein Kinase C Substrate (MARCKS) ELISA Kit
Product Code:
HUDL01796
Size:
96 Assays
Target:
MARCKS
Synonyms:
80K-L, MACS, MRACKS, PKCSL, PRKCSL, Protein kinase C substrate, 80 kDa protein, light chain
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
