Human N-Acylsphingosine Amidohydrolase 1 (ASAH1) ELISA Kit
The Human N-Acylsphingosine Amidohydrolase-1 (ASAH1) ELISA Kit is a powerful tool for the accurate measurement of ASAH1 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it an invaluable asset for various research applications.ASAH1 is a key enzyme involved in sphingolipid metabolism, playing a critical role in maintaining cellular homeostasis and influencing cell signaling processes. Dysregulation of ASAH1 has been linked to a variety of diseases, including cancer, metabolic disorders, and neurological conditions.
By measuring ASAH1 levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the Human ASAH1 ELISA Kit offers a comprehensive solution for studying ASAH1 biology and its implications in health and disease. Its user-friendly design and robust performance make it a must-have tool for researchers working in the fields of biochemistry, cell biology, and drug discovery.
Product Name:
Human N-Acylsphingosine Amidohydrolase 1 (ASAH1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
