Human N-Ethylmaleimide Sensitive Factor (NSF) ELISA Kit
The Human N-Ethylmaleimide-Sensitive Factor (NSF) ELISA Kit is a cutting-edge tool designed for precise detection of NSF levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit ensures accurate and repeatable results, making it the perfect choice for a wide array of research applications.NSF is a critical protein involved in membrane fusion and vesicle trafficking processes, playing a crucial role in cellular function.
Dysregulation of NSF has been implicated in various diseases, including neurodegenerative disorders and immune system dysfunction, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Don't miss out on the opportunity to advance your research with the Human N-Ethylmaleimide-Sensitive Factor (NSF) ELISA Kit. Order yours today and uncover new insights into the intricate mechanisms of cellular biology.
Product Name:
Human N-Ethylmaleimide Sensitive Factor (NSF) ELISA Kit
Product Code:
HUDL02098
Size:
96 Assays
Target:
NSF
Synonyms:
SKD2, N-Ethylmaleimide Sensitive Fusion Protein, Vesicle-fusing ATPase, Vesicular-fusion protein NSF
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
