Human N-myc Downstream Regulated Gene 2 (NDRG2) ELISA Kit
The Human NDRG2 ELISA Kit is specifically designed for the precise measurement of N-Myc downstream-regulated gene 2 (NDRG2) levels in human samples including serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit offers reliable and reproducible results, making it an invaluable tool for a variety of research applications.NDRG2 is a key regulator in various cellular processes, including cell growth, differentiation, and stress responses. Dysregulation of NDRG2 has been associated with cancer, neurodegenerative diseases, and other pathological conditions, highlighting its potential as a biomarker for disease diagnosis and therapeutic development.
This ELISA kit provides researchers with a powerful tool to study the role of NDRG2 in disease pathogenesis and to explore potential targeted therapies. Its ease of use and accuracy make it suitable for both academic and clinical research settings. Unlock the potential of NDRG2 research with the Human NDRG2 ELISA Kit.
Product Name:
Human N-myc Downstream Regulated Gene 2 (NDRG2) ELISA Kit
Product Code:
HUDL02002
Size:
96 Assays
Target:
NDRG2
Synonyms:
SYLD, N-myc downstream-regulated gene 2 protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
