Human NACHT, LRR and PYD Domains-Containing Protein 3 (NLRP3) ELISA Kit
The Human NLRP3 (NACHT, LRR, and PYD domains-containing protein 3) ELISA Kit is specifically designed for the precise measurement of NLRP3 levels in human samples including serum, plasma, and cell culture supernatants. This kit boasts a high level of sensitivity and specificity, ensuring accurate and consistent results that are crucial for various research applications. NLRP3 is a key protein involved in inflammasome activation, playing a critical role in the immune response and inflammatory processes.
Dysregulation of NLRP3 has been linked to various inflammatory diseases such as autoimmune disorders, metabolic disorders, and neurodegenerative conditions. As a fundamental biomarker in understanding these diseases and developing potential treatment strategies, the Human NLRP3 ELISA Kit provides researchers with a valuable tool for their studies.
Product Name:
Human NACHT, LRR and PYD Domains-Containing Protein 3 (NLRP3) ELISA Kit
Product Code:
HUDL02047
Size:
96 Assays
Target:
NLRP3
Synonyms:
PYRIN-containing APAF1-like protein 1, Cryopyrin, Cold autoinflammatory syndrome 1 protein, Angiotensin/vasopressin receptor AII/AVP-like, Caterpiller protein 1.1(CLR1.1)
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
