Human NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) ELISA Kit
The Human NADH dehydrogenase (ubiquinone) Fe-S protein 1 (NDUFS1) ELISA Kit is a highly accurate and reliable tool for measuring levels of NDUFS1 in human samples, including serum, plasma, and cell culture supernatants. NDUFS1 is a key protein involved in the electron transport chain of mitochondria, playing a critical role in energy production within cells.This ELISA kit offers high sensitivity and specificity, enabling researchers to obtain precise and reproducible results for a variety of research applications. By accurately quantifying NDUFS1 levels, this kit can provide valuable insights into cellular metabolism, mitochondrial dysfunction, and related diseases such as mitochondrial disorders, neurodegenerative diseases, and metabolic disorders.
With its user-friendly protocol and robust performance, the Human NDUFS1 ELISA Kit is an essential tool for researchers studying mitochondria, energy metabolism, and various pathologies associated with mitochondrial dysfunction. Order yours today and unlock new possibilities in your research endeavors.
Product Name:
Human NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
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