Human NAGLU (N-Acetyl Alpha-D-Glucosaminidase) ELISA Kit
The Human NAGLU (N-Acetyl-alpha-D-glucosaminidase) ELISA Kit is specifically designed for the precise measurement of NAGLU levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for various research applications. NAGLU is an important enzyme involved in the breakdown of glycosaminoglycans, playing a crucial role in lysosomal degradation.
Dysregulation of NAGLU has been linked to lysosomal storage disorders and other related diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions. With its reliable performance and accuracy, the Human NAGLU ELISA Kit is an essential tool for researchers looking to investigate the role of NAGLU in disease pathogenesis and explore new avenues for diagnosis and treatment.
Product Name:
Human NAGLU (N-Acetyl Alpha-D-Glucosaminidase) ELISA Kit
SKU:
HUES02092
Target:
Human NAGLU (N-Acetyl Alpha-D-Glucosaminidase)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NAGLU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NAGLU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NAGLU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NAGLU. You can calculate the concentration of Human NAGLU in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
96-112
90-105
Average (%)
101
104
97
1:4
Range (%)
91-106
81-91
87-100
Average (%)
97
87
92
1:8
Range (%)
90-102
85-100
85-96
Average (%)
95
91
91
1:16
Range (%)
93-105
86-96
82-93
Average (%)
98
91
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
85-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
3.07
8.61
0.96
2.98
8.66
Standard deviation
0.06
0.14
0.41
0.06
0.12
0.27
C V (%)
6.06
4.56
4.76
6.25
4.03
3.12
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NAGLU concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NAGLU in samples. No significant cross-reactivity or interference between Human NAGLU and analogues was observed.
