Human NAIP (Neuronal Apoptosis Inhibitory Protein) CLIA Kit
The Human NAIP (Neuronal Apoptosis Inhibitory Protein) CLIA Kit is a cutting-edge assay designed for the precise measurement of NAIP levels in human samples including serum, plasma, and cell culture supernatants. This innovative kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.NAIP is a key protein involved in the regulation of neuronal apoptosis, playing a critical role in protecting neurons from cell death.
Dysregulation of NAIP has been linked to various neurodegenerative disorders, highlighting its importance as a potential therapeutic target and biomarker for disease progression.By using the Human NAIP CLIA Kit, researchers can gain valuable insights into the role of NAIP in neuronal health and disease, paving the way for the development of novel treatments and diagnostic tools.Upgrade your research with the Human NAIP CLIA Kit and uncover the secrets of neuronal apoptosis regulation.
Product Name:
Human NAIP (Neuronal Apoptosis Inhibitory Protein) CLIA Kit
SKU:
HUES01048
Target:
Human NAIP (Neuronal Apoptosis Inhibitory Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NAIP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NAIP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NAIP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NAIP. You can calculate the concentration of Human NAIP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-114
88-104
87-101
Average (%)
104
95
94
1:4
Range (%)
100-116
101-116
95-107
Average (%)
108
107
102
1:8
Range (%)
96-110
86-97
87-100
Average (%)
102
91
92
1:16
Range (%)
90-104
94-105
85-99
Average (%)
98
99
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
92
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.51
195.13
694.08
102.32
191.48
711.26
Standard deviation
9.52
20.31
67.4
13.21
21.37
60.53
C V (%)
9.2
10.41
9.71
12.91
11.16
8.51
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NAIP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NAIP in samples. No significant cross-reactivity or interference between Human NAIP and analogues was observed.
