The Human NEF3 (Neurofilament 3) ELISA Kit is specifically designed for the accurate quantification of NEF3 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and reproducible results for various research purposes.NEF3, also known as neurofilament light protein, is a key component of the neuronal cytoskeleton and is commonly used as a biomarker for neurodegenerative diseases such as Alzheimer's and Parkinson's.
Monitoring NEF3 levels can provide valuable insights into disease progression, treatment efficacy, and potential therapeutic targets in the field of neuroscience research.Overall, the Human NEF3 ELISA Kit is a valuable tool for scientists and researchers studying neurodegenerative disorders and exploring new strategies for diagnosis and treatment. Its precision and reliability make it an essential addition to any laboratory conducting cutting-edge research in the field of neuroscience.
Product Name:
Human NEF3 (Neurofilament 3) ELISA Kit
SKU:
HUES01885
Target:
Human NEF3 (Neurofilament 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NEF3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NEF3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NEF3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NEF3. You can calculate the concentration of Human NEF3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
84-99
98-111
Average (%)
103
91
103
1:4
Range (%)
92-104
82-91
87-102
Average (%)
97
87
94
1:8
Range (%)
89-102
86-96
87-100
Average (%)
96
91
93
1:16
Range (%)
88-101
85-96
85-99
Average (%)
94
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
93
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.42
147.8
462.67
48.4
144.11
503.78
Standard deviation
2.59
6.78
22.44
3.24
7.55
15.11
C V (%)
5.14
4.59
4.85
6.69
5.24
3.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NEF3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NEF3 in samples. No significant cross-reactivity or interference between Human NEF3 and analogues was observed.
