Human NEFL (Neurofilament, Light Polypeptide) CLIA Kit
The Human NEFL (Neurofilament Light Polypeptide) CLIA Kit is a cutting-edge assay designed for the precise quantification of NEFL levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts superior sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.NEFL, also known as Neurofilament Light Polypeptide, is a key protein involved in the structural integrity of neurons and axons. Elevated levels of NEFL have been linked to various neurological disorders, including Alzheimer's disease, multiple sclerosis, and ALS.
The Human NEFL CLIA Kit provides researchers with a valuable tool for studying these conditions and exploring potential therapeutic interventions.Overall, the Human NEFL CLIA Kit is a reliable and effective solution for researchers seeking to investigate the role of NEFL in neurodegenerative diseases and advance our understanding of neurological disorders.
Product Name:
Human NEFL (Neurofilament, Light Polypeptide) CLIA Kit
SKU:
HUES00467
Target:
Human NEFL (Neurofilament, Light Polypeptide)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NEFL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NEFL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NEFL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NEFL. You can calculate the concentration of Human NEFL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
95-108
94-109
Average (%)
93
101
102
1:4
Range (%)
89-101
101-116
89-100
Average (%)
96
107
95
1:8
Range (%)
96-113
90-105
89-102
Average (%)
104
96
95
1:16
Range (%)
85-98
87-99
101-114
Average (%)
92
94
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
100
EDTA plasma (n=5)
96-111
104
Cell culture media (n=5)
97-108
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
42.81
110.42
359.68
45.57
109.06
388.0
Standard deviation
4.76
8.26
23.49
5.5
13.02
28.75
C V (%)
11.12
7.48
6.53
12.07
11.94
7.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NEFL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NEFL in samples. No significant cross-reactivity or interference between Human NEFL and analogues was observed.
