The Human Neogenin-1 (NEO1) ELISA Kit is specifically designed for the accurate measurement of neogenin-1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.Neogenin-1 is a cell surface protein that plays a vital role in various cellular processes such as cell adhesion, migration, and axon guidance. Dysregulation of neogenin-1 has been associated with diseases like cancer, neurological disorders, and developmental abnormalities, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With the Human Neogenin-1 (NEO1) ELISA Kit, researchers can accurately quantify neogenin-1 levels in human samples, allowing for in-depth analysis of its role in disease pathogenesis and potential drug targeting strategies. Trust Assay Genie for reliable and high-quality ELISA kits to advance your research endeavors.
Product Name:
Human Neogenin 1 (NEO1) ELISA Kit
Product Code:
HUDL02011
Size:
96 Assays
Target:
NEO1
Synonyms:
NGN, IGDCC2, Immunoglobulin Superfamily,DCC Subclass Member 2
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
