The Human NEP (Neprilysin) ELISA Kit is specifically developed for the precise measurement of neprilysin levels in human biological samples including serum, plasma, and cell culture supernatants. This ELISA kit is known for its high sensitivity and specificity, ensuring accurate and reproducible results, making it a valuable tool for various research applications.Neprilysin is an important enzyme involved in the degradation of peptides, including amyloid beta, which is implicated in Alzheimer's disease.
Elevated levels of neprilysin have been linked to various diseases such as Alzheimer's, heart failure, and diabetes, making it a key biomarker for studying these conditions and exploring potential treatment strategies.Overall, the Human NEP ELISA Kit provides researchers with a reliable and efficient method for quantifying neprilysin levels, facilitating in-depth investigations into the role of this enzyme in health and disease.
Product Name:
Human NEP (Neprilysin) ELISA Kit
SKU:
HUES01937
Target:
Human NEP (Neprilysin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NEP. You can calculate the concentration of Human NEP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
97-110
96-112
Average (%)
99
105
103
1:4
Range (%)
91-104
84-97
82-93
Average (%)
98
91
88
1:8
Range (%)
88-102
85-94
90-104
Average (%)
94
89
95
1:16
Range (%)
87-101
81-92
81-94
Average (%)
94
86
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
102
EDTA plasma (n=5)
96-112
102
Cell culture media (n=5)
94-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.49
1.16
3.64
0.53
1.07
3.7
Standard deviation
0.03
0.06
0.18
0.03
0.05
0.12
C V (%)
6.12
5.17
4.95
5.66
4.67
3.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NEP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NEP in samples. No significant cross-reactivity or interference between Human NEP and analogues was observed.
