The Human NES1 (Nesfatin-1) CLIA Kit is specifically designed for the quantitative measurement of Nesfatin-1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and precision, ensuring accurate and reproducible results for a variety of research purposes.Nesfatin-1 is a peptide hormone known for its role in regulating appetite and energy metabolism.
It also plays a critical role in various physiological processes, making it a valuable biomarker for studying conditions such as obesity, diabetes, and metabolic disorders. The Human NES1 CLIA Kit provides researchers with a reliable tool for investigating the role of Nesfatin-1 in these conditions and exploring potential therapeutic interventions.
Product Name:
Human NES1 (Nesfatin 1) CLIA Kit
SKU:
HUES01223
Target:
Human NES1 (Nesfatin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NES1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NES1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NES1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NES1. You can calculate the concentration of Human NES1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-112
85-101
85-100
Average (%)
104
92
92
1:4
Range (%)
97-114
95-113
84-100
Average (%)
105
103
91
1:8
Range (%)
94-108
99-114
96-111
Average (%)
101
104
104
1:16
Range (%)
83-99
88-100
92-107
Average (%)
90
95
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-109
99
EDTA plasma (n=5)
90-102
96
Cell culture media (n=5)
95-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.65
51.53
214.97
22.1
51.63
200.67
Standard deviation
3.0
3.99
16.57
1.82
5.96
18.16
C V (%)
12.68
7.74
7.71
8.24
11.54
9.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NES1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NES1 in samples. No significant cross-reactivity or interference between Human NES1 and analogues was observed.
