SynonymsSYNE2,NUA,NUANCE,SYNE-2,Synaptic nuclear envelope protein 2,Nuclear envelope spectrin repeat protein 2,Nucleus and actin connecting element protein
ReactivityHuman
Sample TypeTissue Homogenates And Other Biological Fluids
SKU:
HUDL02015
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8WXH0
Sensitivity:
0.112ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SYNE2,NUA,NUANCE,SYNE-2,Synaptic nuclear envelope protein 2,Nuclear envelope spectrin repeat protein 2,Nucleus and actin connecting element protein
The Human Nesprin-2 (Nesp2) ELISA Kit is specifically designed for the precise measurement of Nesprin-2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and reproducible results for various research purposes.Nesprin-2 is a critical protein linked to cellular structure and function, serving as a key component in maintaining nuclear shape and positioning within cells.
Dysregulation of Nesprin-2 has been associated with a range of diseases, including muscular dystrophies and cancer. Studying Nesprin-2 levels can provide valuable insights into disease mechanisms and potential therapeutic strategies.Overall, the Human Nesprin-2 (Nesp2) ELISA Kit is an indispensable tool for researchers seeking to explore the role of Nesprin-2 in health and disease, offering reliable and high-quality data for their investigations.
Product Name:
Human Nesprin 2 (Nesp2) ELISA Kit
Product Code:
HUDL02015
Size:
96 Assays
Target:
Nesp2
Synonyms:
SYNE2, NUA, NUANCE, SYNE-2, Synaptic nuclear envelope protein 2, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
91-101
96
Heparin plasma (n=5)
91-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
