Human Neurofilament, Heavy Polypeptide (NEFH) ELISA Kit
The Human Neurofilament Heavy Polypeptide (NEFH) ELISA Kit is a powerful tool designed for the accurate quantification of neurofilament heavy polypeptide levels in human serum, plasma, and cell culture supernatants. This ELISA kit offers exceptional sensitivity and specificity to ensure precise and reproducible results, making it an excellent choice for a variety of research applications.Neurofilament heavy polypeptide is a key component of the neuronal cytoskeleton, playing a crucial role in maintaining neuronal structure and function.
Elevated levels of neurofilament heavy polypeptide have been linked to neurodegenerative diseases such as Alzheimer's and Parkinson's, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With the Human Neurofilament Heavy Polypeptide (NEFH) ELISA Kit, researchers can accurately measure neurofilament heavy polypeptide levels in biological samples, providing valuable insights into neurodegenerative processes and enabling the development of novel diagnostic and therapeutic strategies.
Product Name:
Human Neurofilament, Heavy Polypeptide (NEFH) ELISA Kit
Product Code:
HUDL02005
Size:
96 Assays
Target:
NEFH
Synonyms:
NFH, 200 kDa neurofilament protein, Neurofilament triplet H protein
Detection Method:
Sandwich
Range:
15.625-1,000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
