Human Neutrophil Activating Protein 2 (NAP2) ELISA Kit
The Human Neutrophil Activating Protein 2 (NAP2) ELISA Kit is a highly reliable and precise tool for the quantitative detection of NAP2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.NAP2 is a significant protein involved in the activation of neutrophils, key players in the body's immune response. By measuring NAP2 levels, researchers can gain valuable insights into the inflammatory processes and immune system function.
This makes the Human NAP2 ELISA Kit essential for studying conditions related to inflammation, infection, and autoimmune disorders, and for developing potential therapeutic interventions.Overall, the Human NAP2 ELISA Kit is a powerful tool for researchers looking to delve deeper into the role of NAP2 in immune responses and inflammatory pathways, offering reliable and precise measurements to support their research efforts.
Product Name:
Human Neutrophil Activating Protein 2 (NAP2) ELISA Kit
Product Code:
HUDL01985
Size:
96 Assays
Target:
DL-NAP2-Hu
Synonyms:
Nucleosome assembly protein 2, NAP-2
Detection Method:
Sandwich
Range:
31.2-2000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
