Human NFYC (Nuclear Transcription Factor Y Gamma) CLIA Kit
The Human NFYC (Nuclear Transcription Factor Y, Gamma) CLIA Kit is a powerful tool for the quantitative measurement of NFYC levels in human samples. This kit offers high sensitivity and accuracy, allowing for precise and reliable results in serum, plasma, and cell culture supernatants. NFYC is a key transcription factor that plays a vital role in gene regulation and cell growth. Dysregulation of NFYC has been linked to various diseases, including cancer and immune disorders, making it a valuable biomarker for research and diagnostic purposes.
With its easy-to-use format and quick results, the Human NFYC CLIA Kit is an essential tool for scientists studying transcriptional regulation and its implications in disease pathology. Order now and unlock the potential of NFYC in your research endeavors.
Product Name:
Human NFYC (Nuclear Transcription Factor Y Gamma) CLIA Kit
SKU:
HUES01186
Target:
Human NFYC (Nuclear Transcription Factor Y Gamma)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NFYC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NFYC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NFYC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NFYC. You can calculate the concentration of Human NFYC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-98
100-115
100-116
Average (%)
91
105
108
1:4
Range (%)
94-108
93-109
98-113
Average (%)
101
99
105
1:8
Range (%)
94-105
100-117
93-108
Average (%)
100
108
100
1:16
Range (%)
84-96
86-99
103-119
Average (%)
91
91
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
103-114
108
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
84-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
196.93
428.3
1860.17
199.94
397.04
1977.06
Standard deviation
25.56
44.97
187.88
17.01
39.51
156.78
C V (%)
12.98
10.5
10.1
8.51
9.95
7.93
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NFYC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NFYC in samples. No significant cross-reactivity or interference between Human NFYC and analogues was observed.
