The Human NGB (Neuroglobin) ELISA Kit is specifically designed for the precise measurement of neuroglobin levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, providing researchers with accurate and reliable results for a variety of research applications.Neuroglobin, a key protein in the brain and nervous system, plays a crucial role in cell survival and oxygen homeostasis. Dysregulation of neuroglobin has been linked to various neurological disorders such as stroke, Alzheimer's disease, and Parkinson's disease, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.
Overall, the Human NGB ELISA Kit offers a valuable tool for researchers seeking to explore the role of neuroglobin in neurological health and disease, with the potential to advance our understanding and treatment of related disorders.
Product Name:
Human NGB (Neuroglobin) ELISA Kit
SKU:
HUES02744
Target:
Human NGB (Neuroglobin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NGB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NGB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NGB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NGB. You can calculate the concentration of Human NGB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-98
87-101
83-96
Average (%)
90
93
90
1:4
Range (%)
87-102
86-97
84-94
Average (%)
94
91
89
1:8
Range (%)
91-104
79-92
82-92
Average (%)
98
86
87
1:16
Range (%)
92-107
84-96
89-100
Average (%)
98
89
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-103
96
EDTA plasma (n=5)
86-96
91
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.48
4.39
0.56
1.49
4.27
Standard deviation
0.03
0.07
0.16
0.03
0.06
0.23
C V (%)
5.45
4.73
3.64
5.36
4.03
5.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NGB concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NGB in samples. No significant cross-reactivity or interference between Human NGB and analogues was observed.
