The Human NNE (Non-Neuronal Enolase) CLIA Kit is a powerful tool for the accurate measurement of NNE levels in human samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it suitable for a variety of research applications.Non-Neuronal Enolase is an important enzyme involved in glycolysis and cell metabolism. It has been shown to play a role in various diseases such as cancer, autoimmune disorders, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
Overall, the Human NNE CLIA Kit offers researchers a precise and efficient method for quantifying NNE levels in human samples, providing valuable insights into the role of this enzyme in different disease states and opening up new avenues for research and drug development.
Product Name:
Human NNE (Non-Neuronal Enolase) CLIA Kit
SKU:
HUES00718
Target:
Human NNE (Non-Neuronal Enolase)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NNE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NNE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NNE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NNE. You can calculate the concentration of Human NNE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
91-104
88-100
Average (%)
99
96
95
1:4
Range (%)
86-100
91-105
96-111
Average (%)
91
98
102
1:8
Range (%)
91-102
100-115
96-111
Average (%)
97
105
103
1:16
Range (%)
89-100
100-113
103-117
Average (%)
94
107
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
93-107
101
Cell culture media (n=5)
89-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
188.62
574.34
1448.18
176.8
549.92
1558.55
Standard deviation
23.54
67.2
113.83
17.75
57.3
139.96
C V (%)
12.48
11.7
7.86
10.04
10.42
8.98
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NNE concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NNE in samples. No significant cross-reactivity or interference between Human NNE and analogues was observed.
