The Human NOG (Noggin) ELISA Kit is specifically designed for the precise measurement of noggin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, which makes it perfect for various research applications.Noggin is a key regulator of bone morphogenetic proteins, playing a critical role in embryonic development and adult tissue homeostasis. Dysregulation of noggin has been linked to various diseases, including skeletal disorders and cancer.
Therefore, detecting noggin levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With the Human NOG ELISA Kit, researchers can easily quantify noggin levels in biological samples, leading to a better understanding of its function and potential implications in disease pathology. This kit is a valuable tool for researchers studying bone metabolism, stem cell biology, and developmental biology.
Product Name:
Human NOG (Noggin) ELISA Kit
SKU:
HUES02888
Target:
Human NOG (Noggin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NOG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NOG. You can calculate the concentration of Human NOG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
90-104
91-106
Average (%)
98
96
98
1:4
Range (%)
90-100
80-95
87-103
Average (%)
95
87
94
1:8
Range (%)
89-100
82-95
89-101
Average (%)
94
88
94
1:16
Range (%)
90-105
86-98
81-94
Average (%)
97
91
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
96
EDTA plasma (n=5)
85-98
90
Cell culture media (n=5)
87-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
370.81
1171.82
3307.52
394.62
1105.13
3627.69
Standard deviation
22.4
58.24
168.35
24.82
59.23
117.17
C V (%)
6.04
4.97
5.09
6.29
5.36
3.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NOG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NOG in samples. No significant cross-reactivity or interference between Human NOG and analogues was observed.
