The Human NPYR1 (Neuropeptide Y Receptor Y1) CLIA Kit is a powerful tool for accurate detection of NPYR1 levels in human samples. This kit boasts high sensitivity and specificity, delivering reliable and reproducible results for a variety of research applications.NPYR1 is a key receptor involved in the regulation of neuropeptide Y, a neurotransmitter that plays a role in a wide range of physiological processes including appetite regulation, stress response, and circadian rhythm control.
Dysregulation of NPYR1 has been implicated in various disorders such as obesity, anxiety, and cardiovascular diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Whether you are studying the role of neuropeptide Y signaling in disease pathogenesis or exploring new therapeutic targets, the Human NPYR1 CLIA Kit is an essential tool for your research needs.
Product Name:
Human NPYR1 (Neuropeptide Y Receptor Y1) CLIA Kit
SKU:
HUES00497
Target:
Human NPYR1 (Neuropeptide Y Receptor Y1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NPYR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NPYR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NPYR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NPYR1. You can calculate the concentration of Human NPYR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-99
100-114
96-110
Average (%)
91
107
102
1:4
Range (%)
89-105
90-104
87-99
Average (%)
96
98
93
1:8
Range (%)
86-98
86-96
96-108
Average (%)
92
91
103
1:16
Range (%)
90-105
92-104
85-99
Average (%)
96
98
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
102
EDTA plasma (n=5)
95-107
100
Cell culture media (n=5)
92-106
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
198.13
446.06
1679.17
180.57
476.0
1754.83
Standard deviation
20.51
45.77
145.92
16.29
38.22
195.14
C V (%)
10.35
10.26
8.69
9.02
8.03
11.12
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NPYR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NPYR1 in samples. No significant cross-reactivity or interference between Human NPYR1 and analogues was observed.
