The Human NPYR2 (Neuropeptide Y receptor Y2) CLIA Kit is specifically designed for the precise measurement of Neuropeptide Y receptor Y2 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and selectivity, ensuring accurate and reproducible results for a variety of research applications.Neuropeptide Y receptor Y2 is a key receptor involved in regulating various physiological processes such as appetite control, stress response, and cardiovascular function.
Dysregulation of this receptor has been implicated in conditions like obesity, anxiety disorders, and metabolic diseases, highlighting its importance as a potential therapeutic target and biomarker for disease management.Overall, the Human NPYR2 CLIA Kit provides researchers with a reliable tool for studying the role of Neuropeptide Y receptor Y2 in health and disease, paving the way for innovative research and new treatment strategies.
Product Name:
Human NPYR2 (Neuropeptide Y Receptor Y2) CLIA Kit
SKU:
HUES00503
Target:
Human NPYR2 (Neuropeptide Y Receptor Y2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NPYR2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NPYR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NPYR2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NPYR2. You can calculate the concentration of Human NPYR2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-111
94-109
89-104
Average (%)
105
102
96
1:4
Range (%)
92-106
85-96
86-101
Average (%)
97
91
93
1:8
Range (%)
91-103
96-108
86-100
Average (%)
96
101
93
1:16
Range (%)
100-114
86-99
99-113
Average (%)
108
92
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
104
EDTA plasma (n=5)
96-107
101
Cell culture media (n=5)
99-117
107
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
212.33
488.78
1403.63
229.52
452.8
1393.13
Standard deviation
24.91
40.62
136.15
23.55
47.05
151.29
C V (%)
11.73
8.31
9.7
10.26
10.39
10.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NPYR2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NPYR2 in samples. No significant cross-reactivity or interference between Human NPYR2 and analogues was observed.
