The Human NRG-3 (Neuregulin-3) CLIA Kit from AssayGenie is a cutting-edge tool for the precise measurement of Neuregulin-3 levels in human samples. This kit offers exceptional sensitivity and accuracy, ensuring dependable and consistent results that are perfect for various research needs.Neuregulin-3 is a vital protein that plays a crucial role in various physiological processes, including neural development and cardiac function. Dysregulation of Neuregulin-3 has been linked to several illnesses, such as schizophrenia, bipolar disorder, and cardiovascular diseases, making it a key biomarker for further investigation and potential treatment options.
Upgrade your research capabilities with the Human NRG-3 (Neuregulin-3) CLIA Kit from AssayGenie and gain valuable insights into the role of Neuregulin-3 in human health and disease. Order now and take your studies to the next level.
Product Name:
Human NRG-3 (Neuregulin 3) CLIA Kit
SKU:
HUES00540
Target:
Human NRG-3 (Neuregulin 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NRG-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NRG-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NRG-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NRG-3. You can calculate the concentration of Human NRG-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
88-98
101-118
Average (%)
107
93
108
1:4
Range (%)
91-107
92-102
92-105
Average (%)
98
97
100
1:8
Range (%)
86-101
92-105
95-112
Average (%)
93
97
102
1:16
Range (%)
94-107
97-112
93-105
Average (%)
99
103
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
106
EDTA plasma (n=5)
88-101
93
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
106.16
243.13
742.12
111.89
222.12
807.71
Standard deviation
12.42
24.17
74.14
11.84
22.59
56.14
C V (%)
11.7
9.94
9.99
10.58
10.17
6.95
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NRG-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NRG-3 in samples. No significant cross-reactivity or interference between Human NRG-3 and analogues was observed.
