The Human NRG-4 (Neuregulin-4) CLIA Kit is a cutting-edge solution for the precise measurement of NRG-4 levels in human samples such as serum, plasma, and cell culture supernatants. With superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an indispensable tool for a wide array of research studies.NRG-4, a member of the neuregulin family, plays a pivotal role in various biological processes, including cardiovascular development, neuronal function, and cancer progression.
By detecting NRG-4 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and explore potential therapeutic strategies.Whether investigating cardiovascular diseases, neurological disorders, or cancer biology, the Human NRG-4 CLIA Kit provides researchers with a reliable and efficient platform to advance their understanding of NRG-4 biology and its implications in human health. Order your kit today and accelerate your research endeavors.
Product Name:
Human NRG-4 (Neuregulin 4) CLIA Kit
SKU:
HUES00534
Target:
Human NRG-4 (Neuregulin 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NRG-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NRG-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NRG-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NRG-4. You can calculate the concentration of Human NRG-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-109
92-108
97-113
Average (%)
104
99
105
1:4
Range (%)
91-104
95-109
92-104
Average (%)
97
102
97
1:8
Range (%)
83-97
91-103
90-106
Average (%)
90
98
98
1:16
Range (%)
100-113
94-109
89-102
Average (%)
107
101
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
103
EDTA plasma (n=5)
95-110
102
Cell culture media (n=5)
93-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.13
4.73
0.52
1.21
4.79
Standard deviation
0.06
0.12
0.36
0.06
0.1
0.34
C V (%)
12.0
10.62
7.61
11.54
8.26
7.1
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NRG-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NRG-4 in samples. No significant cross-reactivity or interference between Human NRG-4 and analogues was observed.
