The Human NRP2 (Neuropilin-2) ELISA Kit is a research tool designed for the precise measurement of neuropilin-2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.Neuropilin-2 is a key protein involved in various biological processes, including angiogenesis, axon guidance, and immune response modulation. Its dysregulation has been implicated in a range of diseases, including cancer, autoimmune disorders, and neurological conditions, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.
For researchers investigating the role of neuropilin-2 in health and disease, the Human NRP2 ELISA Kit is an indispensable tool for reliable and reproducible measurements, enabling a deeper understanding of the complex pathways involving this important protein.
Product Name:
Human NRP2 (Neuropilin 2) ELISA Kit
SKU:
HUES02880
Target:
Human NRP2 (Neuropilin 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NRP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NRP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NRP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NRP2. You can calculate the concentration of Human NRP2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-112
88-101
86-98
Average (%)
105
94
93
1:4
Range (%)
91-102
87-98
87-101
Average (%)
97
92
93
1:8
Range (%)
87-100
86-99
85-97
Average (%)
93
93
91
1:16
Range (%)
91-105
85-101
85-96
Average (%)
98
92
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-98
92
EDTA plasma (n=5)
89-99
94
Cell culture media (n=5)
85-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.18
3.59
0.45
1.12
3.3
Standard deviation
0.03
0.05
0.12
0.02
0.05
0.12
C V (%)
6.38
4.24
3.34
4.44
4.46
3.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NRP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NRP2 in samples. No significant cross-reactivity or interference between Human NRP2 and analogues was observed.
