The Human NSE (Neuron Specific Enolase) CLIA Kit is specifically designed for the accurate measurement of NSE levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.Neuron Specific Enolase is an important biomarker for neuronal damage and degeneration in conditions such as stroke, traumatic brain injury, and neurodegenerative diseases like Alzheimer's and Parkinson's.
Monitoring NSE levels can provide valuable insights into disease progression and response to treatment, making this kit a valuable tool for researchers and healthcare professionals alike. With its user-friendly format and reliable performance, the Human NSE CLIA Kit from Assay Genie is an essential resource for studying neurological disorders and advancing our understanding of brain health.
Product Name:
Human NSE (Neuron-Specific Enolase) CLIA Kit
SKU:
HUES00614
Target:
Human NSE (Neuron-Specific Enolase)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.28 ng/mL
Detection range:
0.47-30 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NSE. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NSE, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NSE. You can calculate the concentration of Human NSE in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-103
99-112
93-107
Average (%)
98
106
101
1:4
Range (%)
86-101
96-107
96-110
Average (%)
92
102
103
1:8
Range (%)
91-106
101-117
94-111
Average (%)
98
109
101
1:16
Range (%)
88-98
97-110
98-110
Average (%)
93
105
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-117
107
EDTA plasma (n=5)
95-110
100
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.49
4.37
10.89
1.35
4.66
11.5
Standard deviation
0.19
0.4
1.21
0.17
0.49
0.7
C V (%)
12.75
9.15
11.11
12.59
10.52
6.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NSE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NSE in samples. No significant cross-reactivity or interference between Human NSE and analogues was observed.
