The Human NT-3 (Neurotrophin-3) CLIA Kit is specially designed for the accurate measurement of neurotrophin-3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Neurotrophin-3 is a critical growth factor that plays a key role in the development and maintenance of the nervous system. It is involved in promoting the survival and differentiation of neurons, making it a crucial biomarker for studying neurological conditions and potential treatment options.
By using the Human NT-3 CLIA Kit, researchers can gain valuable insights into the role of neurotrophin-3 in neurological disorders such as Alzheimer's disease, Parkinson's disease, and spinal cord injuries. With its reliable performance and accuracy, this kit is an essential tool for advancing our understanding of neurotrophin-3 biology and developing innovative therapeutic approaches.
Product Name:
Human NT-3 (Neurotrophin-3) CLIA Kit
SKU:
HUES01045
Target:
Human NT-3 (Neurotrophin-3)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NT-3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NT-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NT-3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NT-3. You can calculate the concentration of Human NT-3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
88-98
90-102
Average (%)
96
93
96
1:4
Range (%)
96-110
91-104
88-100
Average (%)
102
97
95
1:8
Range (%)
99-112
93-108
87-99
Average (%)
105
100
92
1:16
Range (%)
100-118
103-119
98-111
Average (%)
108
109
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
103
EDTA plasma (n=5)
98-116
106
Cell culture media (n=5)
102-118
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.73
84.81
399.67
51.47
80.06
421.55
Standard deviation
4.53
9.77
37.37
5.41
9.54
36.84
C V (%)
8.43
11.52
9.35
10.51
11.92
8.74
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NT-3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NT-3 in samples. No significant cross-reactivity or interference between Human NT-3 and analogues was observed.
