Based on the information in the provided URL, we have the Human NT-4 (Neurotrophin-4) CLIA Kit from Assay Genie. This kit is specifically designed for the precise detection of Neurotrophin-4 levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it a valuable tool for various research applications.Neurotrophin-4, also known as NT-4, is a key neurotrophic factor that plays a crucial role in nerve cell development and survival.
It is involved in processes such as neuronal differentiation, synaptic plasticity, and neuroprotection. Dysregulation of Neurotrophin-4 has been linked to various neurological disorders, making it a valuable biomarker for studying and understanding these conditions.Overall, the Human NT-4 CLIA Kit from Assay Genie provides researchers with a reliable and effective tool for measuring Neurotrophin-4 levels, offering insights into its role in neurological processes and potential therapeutic applications.
Product Name:
Human NT-4 (Neurotrophin 4) CLIA Kit
SKU:
HUES01046
Target:
Human NT-4 (Neurotrophin 4)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NT-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NT-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NT-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NT-4. You can calculate the concentration of Human NT-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
100-115
84-99
Average (%)
96
107
91
1:4
Range (%)
88-101
95-109
99-114
Average (%)
93
102
106
1:8
Range (%)
103-115
95-113
91-104
Average (%)
109
103
98
1:16
Range (%)
92-106
93-107
100-114
Average (%)
97
98
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
104
EDTA plasma (n=5)
99-117
106
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
105.6
267.46
841.29
115.36
251.07
925.33
Standard deviation
9.22
24.5
67.64
11.95
29.0
70.6
C V (%)
8.73
9.16
8.04
10.36
11.55
7.63
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NT-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NT-4 in samples. No significant cross-reactivity or interference between Human NT-4 and analogues was observed.
