The Human NT-4 (Neurotrophin 4) ELISA Kit is meticulously crafted to enable the precise quantitative measurement of Neurotrophin 4 levels in diverse human biological samples.
Neurotrophin 4, an essential neurotrophic factor, plays a pivotal role in neuronal survival, development, and function. Its quantification provides valuable insights into neurobiological processes, neural cell differentiation, and neuroprotective mechanisms crucial for nervous system regulation. Our NT-4 ELISA Kit boasts exceptional sensitivity and specificity, ensuring the accuracy and reproducibility of results. With a focus on stringent quality control measures and manufacturing standards, this kit delivers robust performance and user-friendly protocols ideal for various research endeavors and clinical investigations. Count on Assay Genie's NT-4 ELISA Kit for the precise and reliable quantification of this significant neurotrophic factor in your studies.
Product Name:
Human NT-4 (Neurotrophin 4) ELISA Kit
SKU:
AEES00128
Target:
Human NT-4 (Neurotrophin 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NT-4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NT-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NT-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NT-4. You can calculate the concentration of Human NT-4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-107
84-93
93-101
Average (%)
103
90
97
1:4
Range (%)
94-103
92-101
83-96
Average (%)
100
96
90
1:8
Range (%)
96-106
96-109
84-98
Average (%)
103
102
91
1:16
Range (%)
102-109
94-107
97-109
Average (%)
105
100
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-97
91
EDTA plasma (n=5)
85-98
91
Cell culture media (n=5)
90-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
88.61
228.84
991.82
83.18
227.7
984.95
Standard deviation
4.51
10.32
44.04
4.64
11.23
43.44
C V (%)
5.09
4.51
4.44
5.58
4.93
4.41
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human NT-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NT-4 in samples. No significant cross-reactivity or interference between Human NT-4 and analogues was observed.
