Human Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) ELISA Kit
The Human Nuclear Factor of Activated T Cells Cytoplasmic 2 (NFATC2) ELISA Kit is a highly reliable and sensitive assay designed for the accurate detection of NFATC2 levels in human serum, plasma, and cell culture supernatants. NFATC2 is a key transcription factor that plays a crucial role in the regulation of immune responses and cell growth.This ELISA kit offers high specificity and reproducibility, providing researchers with trustworthy results for a variety of research applications.
With its ability to measure NFATC2 levels, this kit is essential for studying immune system function, inflammatory processes, and the development of potential therapies for diseases associated with NFATC2 dysregulation.Overall, the Human NFATC2 ELISA Kit is a valuable tool for researchers seeking to understand the role of NFATC2 in health and disease, making it a vital component in advancing biomedical research.
Product Name:
Human Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
