Human OIT3 (Oncoprotein Induced Transcript 3) CLIA Kit
The Human OIT3 (Oncoprotein Induced Transcript 3) CLIA Kit is specifically designed for the accurate and sensitive detection of OIT3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.OIT3 is a key protein that plays a crucial role in oncogenesis and tumor progression by promoting cell growth and metastasis. It is known to be upregulated in various cancer types, making it a valuable biomarker for studying cancer development and progression.
This CLIA kit provides researchers with a reliable tool for analyzing OIT3 levels in biological samples, offering insights into its potential role in cancer research and therapy development.Overall, the Human OIT3 CLIA Kit provides researchers with a valuable tool for investigating the role of OIT3 in cancer biology and may contribute to the development of novel therapeutic strategies targeting OIT3-related pathways.
Product Name:
Human OIT3 (Oncoprotein Induced Transcript 3) CLIA Kit
SKU:
HUES00601
Target:
Human OIT3 (Oncoprotein Induced Transcript 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human OIT3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OIT3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OIT3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human OIT3. You can calculate the concentration of Human OIT3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
103-118
96-112
87-102
Average (%)
109
102
93
1:4
Range (%)
101-117
88-98
101-115
Average (%)
109
93
108
1:8
Range (%)
97-115
104-116
85-99
Average (%)
105
110
91
1:16
Range (%)
97-112
92-106
92-107
Average (%)
104
98
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
92-107
98
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
211.39
418.16
1945.97
212.08
378.58
1869.83
Standard deviation
19.36
47.46
185.45
27.1
40.58
207.55
C V (%)
9.16
11.35
9.53
12.78
10.72
11.1
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human OIT3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OIT3 in samples. No significant cross-reactivity or interference between Human OIT3 and analogues was observed.
