The Human OLFM4 (Olfactomedin-4) CLIA Kit is specifically designed for the accurate measurement of Olfactomedin-4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.OLFM4, also known as hGC-1 or GW112, is a protein that plays a critical role in regulating inflammation, cell differentiation, and cancer progression. Elevated levels of Olfactomedin-4 have been associated with various diseases and conditions, including inflammatory disorders, cancer, and gastrointestinal diseases.
Therefore, the Human OLFM4 CLIA Kit serves as a valuable tool for studying Olfactomedin-4's involvement in these pathologies and developing potential therapeutic interventions.Overall, the Human OLFM4 CLIA Kit provides researchers with a precise and efficient method for quantifying Olfactomedin-4 levels in human samples, enabling comprehensive investigations into its biological functions and clinical implications.
Product Name:
Human OLFM4 (Olfactomedin 4) CLIA Kit
SKU:
HUES01137
Target:
Human OLFM4 (Olfactomedin 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human OLFM4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OLFM4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OLFM4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human OLFM4. You can calculate the concentration of Human OLFM4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-115
99-114
95-111
Average (%)
109
108
103
1:4
Range (%)
91-103
96-111
88-102
Average (%)
97
104
94
1:8
Range (%)
90-101
100-113
89-102
Average (%)
96
106
95
1:16
Range (%)
95-110
99-113
91-103
Average (%)
102
104
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
94
EDTA plasma (n=5)
97-112
105
Cell culture media (n=5)
98-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
51.9
124.26
497.52
55.22
123.08
477.9
Standard deviation
6.71
10.66
35.97
6.27
11.15
36.89
C V (%)
12.93
8.58
7.23
11.35
9.06
7.72
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human OLFM4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OLFM4 in samples. No significant cross-reactivity or interference between Human OLFM4 and analogues was observed.
