The Human OPG (Osteoprotegerin) CLIA Kit is specifically designed for the precise measurement of OPG levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.OPG, also known as osteoprotegerin, is a key regulator of bone metabolism and plays a crucial role in regulating bone resorption. Dysregulation of OPG levels has been implicated in various bone diseases such as osteoporosis and osteopetrosis, as well as inflammatory conditions like rheumatoid arthritis.
As such, OPG serves as a valuable biomarker for studying these conditions and developing potential treatment strategies.With its high-quality performance and ease of use, the Human OPG CLIA Kit from Assay Genie is a valuable tool for researchers looking to investigate the role of OPG in bone health and disease. Order your kit today and discover new insights into the complex mechanisms of bone metabolism.
Product Name:
Human OPG (Osteoprotegerin) CLIA Kit
SKU:
HUES00778
Target:
Human OPG (Osteoprotegerin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human OPG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OPG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OPG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human OPG. You can calculate the concentration of Human OPG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-114
101-119
101-117
Average (%)
108
109
107
1:4
Range (%)
96-114
86-100
85-98
Average (%)
104
93
91
1:8
Range (%)
97-113
98-115
101-120
Average (%)
104
106
109
1:16
Range (%)
102-119
94-107
92-106
Average (%)
109
101
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
86-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.2
256.55
668.84
86.9
264.82
654.79
Standard deviation
9.78
28.78
58.26
9.19
20.13
67.51
C V (%)
10.49
11.22
8.71
10.58
7.6
10.31
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human OPG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OPG in samples. No significant cross-reactivity or interference between Human OPG and analogues was observed.
