The Human ORM2 (Orosomucoid 2) CLIA Kit is a reliable tool for the precise measurement of ORM2 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it suitable for various research applications.ORM2, also known as Orosomucoid 2, is a glycoprotein that plays a role in inflammation and immune response regulation. Elevated levels of ORM2 have been linked to various health conditions, including inflammatory diseases, cancer, and metabolic disorders.
Studying ORM2 levels can provide valuable insights into these conditions and aid in the development of potential therapies.Overall, the Human ORM2 CLIA Kit offers researchers a valuable tool for investigating the role of ORM2 in health and disease, with the potential to contribute to advancements in diagnostics and treatment strategies.
Product Name:
Human ORM2 (Orosomucoid 2) CLIA Kit
SKU:
HUES00939
Target:
Human ORM2 (Orosomucoid 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ORM2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ORM2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ORM2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human ORM2. You can calculate the concentration of Human ORM2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-105
95-112
100-115
Average (%)
96
103
107
1:4
Range (%)
95-108
83-95
89-102
Average (%)
101
90
96
1:8
Range (%)
103-118
102-118
91-102
Average (%)
109
108
96
1:16
Range (%)
89-104
89-99
99-116
Average (%)
95
94
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-110
102
EDTA plasma (n=5)
97-115
105
Cell culture media (n=5)
86-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
48.03
111.45
464.81
48.0
102.91
478.42
Standard deviation
5.86
9.27
43.92
4.32
10.25
53.87
C V (%)
12.2
8.32
9.45
9.0
9.96
11.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human ORM2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human ORM2 in samples. No significant cross-reactivity or interference between Human ORM2 and analogues was observed.
