The Human OSMR (Oncostatin M Receptor) CLIA Kit is specifically designed for the accurate detection of OSMR levels in human samples such as serum, plasma, and cell culture supernatants. This high-quality kit offers exceptional sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.The OSMR protein is a key component in the regulation of inflammatory responses and immune system functions. Its role in various diseases including cancer, autoimmune disorders, and neuroinflammatory conditions makes it a valuable biomarker for studying disease mechanisms and developing targeted therapies.
With the Human OSMR CLIA Kit, researchers can confidently measure OSMR levels in human samples, providing valuable insights into disease pathogenesis and potential treatment strategies. Trust in this kit for robust and accurate OSMR detection in your research studies.
Product Name:
Human OSMR (Oncostatin M Receptor) CLIA Kit
SKU:
HUES01192
Target:
Human OSMR (Oncostatin M Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human OSMR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OSMR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OSMR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human OSMR. You can calculate the concentration of Human OSMR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-108
103-119
98-108
Average (%)
99
109
103
1:4
Range (%)
92-104
100-118
99-116
Average (%)
97
108
107
1:8
Range (%)
100-118
93-107
100-115
Average (%)
108
99
107
1:16
Range (%)
98-110
94-110
91-104
Average (%)
104
101
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
85-101
92
Cell culture media (n=5)
93-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
104.61
243.26
725.4
105.97
257.71
754.98
Standard deviation
10.54
28.17
61.08
11.01
28.81
53.68
C V (%)
10.08
11.58
8.42
10.39
11.18
7.11
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human OSMR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OSMR in samples. No significant cross-reactivity or interference between Human OSMR and analogues was observed.
