The Human OSMR (Oncostatin M Receptor) ELISA Kit is a reliable and accurate tool for detecting OSMR levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.The Oncostatin M Receptor (OSMR) is a key protein in the regulation of cell growth, differentiation, and immune responses. It is involved in various diseases including cancer, inflammation, and autoimmune disorders, making it a valuable biomarker for studying these conditions and exploring therapeutic interventions.
With the Human OSMR ELISA Kit, researchers can confidently measure OSMR levels in human samples to advance their understanding of disease mechanisms and potential treatment strategies. Trust in the accuracy and reliability of this kit for your research needs.
Product Name:
Human OSMR (Oncostatin M Receptor) ELISA Kit
SKU:
HUES03173
Target:
Human OSMR (Oncostatin M Receptor)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human OSMR. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OSMR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OSMR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human OSMR. You can calculate the concentration of Human OSMR in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
95-107
96-110
Average (%)
102
101
104
1:4
Range (%)
89-101
81-95
85-97
Average (%)
94
87
90
1:8
Range (%)
89-100
82-92
84-96
Average (%)
95
87
91
1:16
Range (%)
94-110
81-93
85-97
Average (%)
100
88
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
99
EDTA plasma (n=5)
95-109
101
Cell culture media (n=5)
91-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
1.25
3.79
0.51
1.15
4.07
Standard deviation
0.03
0.07
0.14
0.03
0.06
0.2
C V (%)
5.56
5.6
3.69
5.88
5.22
4.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human OSMR concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OSMR in samples. No significant cross-reactivity or interference between Human OSMR and analogues was observed.
