The Human OTR (Oxytocin Receptor) CLIA Kit is specifically designed to accurately detect levels of oxytocin receptor in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit guarantees reliable and reproducible results, making it an invaluable tool for various research purposes. The oxytocin receptor is a critical component in mediating the effects of oxytocin, a hormone involved in social bonding, reproduction, and stress regulation. Dysregulation of the oxytocin receptor has been linked to various disorders such as autism spectrum disorders, anxiety, and depression, highlighting its importance as a potential biomarker for studying and developing treatments for these conditions.
By utilizing the Human OTR CLIA Kit, researchers can gain valuable insights into the role of the oxytocin receptor in different physiological and pathological processes, ultimately advancing our understanding of complex diseases and paving the way for innovative therapeutic interventions.
Product Name:
Human OTR (Oxytocin Receptor) CLIA Kit
SKU:
HUES00887
Target:
Human OTR (Oxytocin Receptor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human OTR. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human OTR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human OTR, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human OTR. You can calculate the concentration of Human OTR in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-110
87-102
93-107
Average (%)
104
94
99
1:4
Range (%)
94-106
87-100
102-119
Average (%)
99
93
110
1:8
Range (%)
98-114
85-100
97-110
Average (%)
106
91
104
1:16
Range (%)
100-115
88-103
97-112
Average (%)
107
95
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-116
107
EDTA plasma (n=5)
95-108
102
Cell culture media (n=5)
87-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.33
268.69
711.8
116.53
284.01
733.17
Standard deviation
10.12
31.03
62.42
12.22
30.16
53.23
C V (%)
9.17
11.55
8.77
10.49
10.62
7.26
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human OTR concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human OTR in samples. No significant cross-reactivity or interference between Human OTR and analogues was observed.
