The Human P-Cadherin (Cadherin-3) Placental CLIA Kit (HU-ES00545) is a highly accurate and reliable assay designed for the precise detection of P-Cadherin levels in human samples. This innovative kit offers high sensitivity and specificity, ensuring dependable and reproducible results for a variety of research applications.P-Cadherin, also known as Cadherin-3, plays a critical role in cell-cell adhesion and tissue organization. Abnormal P-Cadherin expression has been linked to various cancers and developmental disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
With its advanced technology and ease of use, the Human P-Cadherin Placental CLIA Kit (HU-ES00545) is a valuable tool for researchers and clinicians looking to study P-Cadherin levels in human samples accurately and efficiently.
Product Name:
Human P-Cadherin (Cadherin, Placental) CLIA Kit
SKU:
HUES00545
Target:
Human P-Cadherin (Cadherin, Placental)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human P-Cadherin. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human P-Cadherin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human P-Cadherin, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human P-Cadherin. You can calculate the concentration of Human P-Cadherin in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
98-114
86-98
Average (%)
95
105
92
1:4
Range (%)
92-107
99-114
88-100
Average (%)
99
106
93
1:8
Range (%)
86-102
93-108
97-109
Average (%)
93
101
103
1:16
Range (%)
98-114
92-106
94-109
Average (%)
104
98
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
107
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
98-112
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.3
132.83
408.68
56.36
141.5
442.94
Standard deviation
4.69
10.8
25.09
5.28
9.92
27.64
C V (%)
8.97
8.13
6.14
9.37
7.01
6.24
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human P-Cadherin concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human P-Cadherin in samples. No significant cross-reactivity or interference between Human P-Cadherin and analogues was observed.
